Coding

Part:BBa_K3346004:Design

Designed by: Emily Laskey   Group: iGEM20_Rochester   (2020-10-11)


Anti-Estradiol Fab Fragment


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Restriction enzyme sites were removed and sequence was optimized for use in E. coli using Reverse Translate Software available on The Sequence Manipulation Suite.


Source

This sequence comes from (mouse) monoclonal antibody 57-2 anti-17beta-estradiol.

References